Figure 1

Expression of CD11c on pulmonary CD8⁺T cells following RSV infection. Following primary RSV infection lungs, spleens, MLN and blood were harvested from BALB/c mice at the time points indicated. Cells were stained with antibodies to CD3, CD8, and CD11c, CD11b or F4/80. Dot plots show expression of CD11c and CD8 on cells gated for CD3⁺ lymphocytes harvested from A) lungs, B) spleen, MLN and blood. Numbers indicate the percentage of CD8⁺ cells. Quadrant settings were adjusted that less than 1% of CD8⁺ cells stained positive for the isotype control of anti-CD11c-mAb. C) Changes in expression of CD11c, CD11b and F4/80 on CD3⁺ CD8⁺ cells from lungs, spleen, MLN, and blood following RSV infection. Cells were stained with antibodies as indicated and analyzed by FACS. The percentage of CD3⁺8⁺ cells staining positive for CD11c (black squares), CD11b (open circles) or F4/80 (triangles) after subtraction of isotype controls is shown as mean ± SEM (n = 6, *p < 0.05, significant difference versus dpi 0).