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Table 1 Binding of PAO1 and LDH release by 9HTEo- cell lines

From: Relation of exaggerated cytokine responses of CF airway epithelial cells to PAO1 adherence

 

pCEP

pCEP-R

 

LDH (U/ml)

GFP -PA01 (RFU)

LDH (U/ml)

GFP-PA01 (RFU)

PA

8.83 ± 2.5

0.3483 ± 0.12

4.64 ± 0.7*

0.3745 ± 0.123

PA + EGTA

3.77 ± 0.9

ND

2.82 ± 0.0

ND

PA + aGM1

2.36 ± 0.5

0.7764 ± 0.037†

3.3 ± 0.5

0.5489 ± 0.085*†

PA + GM1

2.83 ± 0.0

0.6929 ± 0.049†

1.89 ± 0.0

0.4598 ± 0.090*

PA + C.p.

ND

0.6350 ± 0.091†

ND

0.4359 ± 0.079*

No treatment

4.79 ± 1.0

ND

2.44 ± 0.9 *

ND

EGTA alone

2.36 ± 0.5

ND

4.24 ± 0.5

ND

aGM1 alone

3.3 ± 0.5

ND

2.83 ± 0.9

ND

GM1 alone

2.83 ± 0.9

ND

3.3 ± 0.5

ND

  1. *Different from pCEP congener, p < 0.05
  2. † Different from no treatment, p < 0.05