Figure 2
From: Relation of exaggerated cytokine responses of CF airway epithelial cells to PAO1 adherence
Exogenous aGM1 is incorporated into 9HTEo-pCEP and pCEP-R. pCEP (a-d) and pCEP-R (e-h) cells were incubated with either vehicle (a,c,e,g) or aGM1 (b,d,f,h) and fluorescent staining with either antibody to aGM1 and FITC secondary (b,f) or FITC- conjugated PNA (D,H) was performed. In the untreated state, there is more binding of antibody to aGM1 or PNA to pCEP-R than pCEP cells. After incubation with aGM1, fluorescence patterns are similar for pCEP and pCEP-R cells with antibody to aGM1 (b,f) and PNA (d,h). Micrographs are representative of 3 separate experiments.