Table 1 Optimized conditions for RT-PCR
From: Alveolar macrophages regulate neutrophil recruitment in endotoxin-induced lung injury
| Size of PCR | ||||
|---|---|---|---|---|
| Gene | Primers | Fragments (bp) | Thermocycle Condition | |
| TNF-α | sense | 5'-ACT GAA CTT CGG GGT GAT TG-3' | 334 | 23 cycles; Tm: 57°C |
| antisense | 5'-GTG GGT GAG GAG CAG GTA GT-3' | |||
| IL-1β | sense | 5'-AGC TGC ACT GCA GGC TTC GAG ATG-3' | 339 | 28 cycles; Tm: 68°C |
| antisense | 5'-GAA CTG TGC AGA CTC AAA CTC CAC-3' | |||
| MCP-1 | sense | 5'-TAT GCA GGT CTC TGT CAC GC-3' | 255 | 24 cycles; Tm: 58°C |
| antisense | 5'-TTC CTT ATT GGG GTC AGC AC-3' | |||
| MIP-1β | sense | 5'-CGT GTC TGC CTT CTC TCT CC-3' | 220 | 24 cycles; Tm: 58°C |
| antisense | 5'-CAC AGA TTT GCC TGC CTT TT-3' | |||
| ICAM-1 | sense | 5'-AGG TAT CCA TCC ATC CCA CA-3' | 660 | 22 cycles; Tm: 58°C |
| antisense | 5'-CTT CAG AGG CAG GAA ACA GG-3' | |||
| VCAM-1 | sense | 5'-CGG TCA TGG TCA AGT GTT TG-3' | 570 | 24 cycles; Tm: 57°C |
| antisense | 5'-ACC CTC ATG TAG CCT TGT GG-3' | |||