Figure 8

(A) Representative images of MMP-2 immunostaining in A549 cells cultured with recombinant epimorphin (right panel) or with BL (left panel) after 9 hours of incubation. In the epimorphin-coated slides, plated cells formed some monolayer cell islands in addition to loose sheets of cells, and cells in these cell islands showed strong MMP-2 immunostaining (by the immunoperoxidase method). Scale bars = 30 μm. (B) Via the MMP-2 Biotrack Activity Assay System, MMP-2 activity in the supernatants of A549 cells cultured with recombinant epimorphin was higher than in supernatants of cells cultured with BL or Type IV collagen (P = 0.01).