Figure 1

TLR4 mRNA expression in type II cells. Detection of TLR4 (upper lane) and TLR2 mRNA expression (middle lane) in type II cells after exposure to C. pneumoniae for 2 hours (1) and in non-infected control cells (2) by semiquantitative polymerase chain reaction. GAPDH (lower lane) served as internal control. After 2 hours of incubation with C. pneumoniae TLR4 mRNA expression in type II cells approximately doubled whereas TLR2 mRNA remained unchanged.