Table 1 Effects of N-AcS, DHS and DMS on muscarine-induced reductions in luminal airway area in FVB mice. The number of experiments (lungs/slices), mean airway diameter in μm, and the luminal airway area determined 1 min (1) and 15 min (2) after stimulation with muscarine (Mus) and 1 min (3) and 15 min (4) after repeated stimulation with muscarine or after repeated stimulation with muscarine in combination with N-AcS, DHS or DMS are shown. In all experiments, the muscarine concentration was 10^-6 M. Data are given as means ± S.E.M. Matched pairs (1 vs. 3; 2 vs. 4) were evaluated by Wilcoxon's rank sum test. Differences were considered as statistically significant when p < 0.05 (n.s., not significant).

Mus

Mus

n.s.

n.s.

4/4

206 ± 7

31 ± 6

38 ± 4

38 ± 7

28 ± 3

Mus

Mus/N-AcS 10^-6 M

n.s.

n.s.

6/5

182 ± 7

47 ± 8

42 ± 8

50 ± 8

42 ± 9

Mus

Mus/N-AcS 10^-8 M

n.s.

n.s.

5/5

218 ± 5

36 ± 1

28 ± 5

38 ± 6

26 ± 6

Mus

Mus/N-AcS 10^-10 M

n.s.

n.s.

4/4

212 ± 22

21 ± 8

16 ± 5

26 ± 6

30 ± 5

Mus

Mus/DHS 10^-4 M

n.s.

n.s.

4/6

194 ± 9

42 ± 5

37 ± 7

61 ± 8

45 ± 6

Mus

Mus/DHS 10^-6 M

p < 0.05

p < 0.05

7/7

197 ± 14

45 ± 11

31 ± 7

70 ± 13

50 ± 5

Mus

Mus/DHS 10^-8 M

p < 0.05

p < 0.05

7/9

181 ± 6

39 ± 7

29 ± 6

50 ± 9

47 ± 7

Mus

Mus/DHS 10^-10 M

p < 0.05

p < 0.05

4/8

198 ± 6

34 ± 3

24 ± 3

42 ± 4

40 ± 4

Mus

Mus/DMS 10^-4 M

p < 0.05

p < 0.05

4/7

180 ± 7

30 ± 7

25 ± 6

45 ± 10

41 ± 7

Mus

Mus/DMS 10^-6 M

p < 0.05

p < 0.05

4/7

201 ± 11

48 ± 7

35 ± 8

70 ± 6

54 ± 9

Mus

Mus/DMS 10^-8 M

p < 0.05

p < 0.05

4/7

206 ± 11

31 ± 5

25 ± 4

54 ± 8

50 ± 5

Mus

Mus/DMS 10^-10 M

p < 0.05

p < 0.05

4/6

196 ± 15

43 ± 6

23 ± 4

71 ± 11

52 ± 7