Figure 4
Effect of various treatments on muscarine-mediated reductions in luminal area of peripheral bronchi from FVB mice. The luminal area of peripheral bronchi (expressed in %) was recorded by videomorphometry after application of 10-6 M muscarine alone or after application of 10-6 M muscarine in the presence of (A) 10-6 M La3+ (7 slices from 4 lungs), (B) SKF 96365 (SKF 10-5 M, 9 slices from 4 lungs), (C) 10-6 M La3+ in combination with 10-6 M DHS (7 slices from 4 lungs), or (D) 10-5 M SKF 96365 in combination with 10-6 M DHS (8 slices from 4 lungs). Data are given as means ± S.E.M.E-F) Summary of effects of various treatments on muscarine-mediated reductions in luminal area of peripheral bronchi from FVB mice. Luminal area (expressed in %) determined 1 min (initial contraction, E) and 15 min (sustained contraction, G) after application of 10-6 M muscarine alone or of muscarine in combination with either La3+ (both 10-6 M) or La3+ in combination with DHS or DMS (both 10-8 M). Luminal area (expressed in %) determined 1 min (initial contraction, F) and 15 min (sustained contraction, H) after application of muscarine alone or of muscarine in combination with either SKF 96365 (10-5 M) or SKF 96365 in combination with 10-8 M DHS or DMS. Asterisks indicate p < 0.05 for the comparison with application of muscarine alone (E-F). Daggers indicate p < 0.05 for the comparison with application of muscarine in combination with La3+ (E, G). Data are given as means ± S.E.M.