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Figure 1 | Respiratory Research

Figure 1

From: Activation of the SPHK/S1P signalling pathway is coupled to muscarinic receptor-dependent regulation of peripheral airways

Figure 1

A) Quantitative RT-PCR analysis of SPHK1 expression in PCLS from different mouse strains. The relative expression of SPHK1-mRNA in relation to the house-keeping gene β2 microglobulin is shown. Data are given as means ± S.E.M. of four independent experiments each carried out in triplicate. B) Indirect immunofluorescence studies examining SPHK1 expression in PCLS of FVB-mice. Immunoreactivity for SPHK1 was present in the wall of larger (a) and smaller (b) airways and in the media of pulmonary vessels (arrows in a, b). Consecutive sections (c, d) showing that preabsorption of the SPHK1 antiserum abolished immunolabelling (Pre, d). Bars = 50 μm.

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Respiratory Research

ISSN: 1465-993X

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