Figure 1
Confocal images of rat lung slice co-stained with the LIVE/DEAD®viability/cytotoxicity assay kit for animal cells. A) A 200 μm rat lung slice loaded with 1 μM calcein-AM/4 μM EthD-1 and excited with light of 488 nm (green emission) to show viable cells. B) The same 200 μm rat lung slice loaded with 1 μM calcein-AM/4 μM EthD-1 and excited with light of 532 nm, (red emission) to show the potential dead cells. C) Overlay of images (A) and (B) showing that the majority of the cells are alive. D) A 200 μm rat lung slice loaded with 1 μM calcein-AM/4 μM EthD-1 following a 15 minute treatment with 1% Triton X-100 and then excited with light of 488 nm (green emission) to show viable cells. E) The same 200 μm rat lung slice loaded with 1 μM calcein-AM/4 μM EthD-1 following a 15 minute treatment with 1% Triton X-100 and then excited with light of 532 nm, (red emission) to show the potential dead cells. F) Overlay of images (D) and (E) showing that no live cells remain following treatment with Triton X-100.