Figure 4

The levels of RhoA protein in the bronchi obtained from the sensitized control (Control) and repeatedly ovalbumin (OA)-challenged (OA-challenged) mice. (A) Typical immunoblots. Lane 1; Control, lane 2; OA-challenged, and GAPDH; glyceraldehyde-3-phosphate dehydrogenase. The bands were analyzed by a densitometer and normalized by the intensity of corresponding GAPDH band, and the data are summarized in B. Each column represents the mean ± S.E. from 5 experiments. The expression level of RhoA protein in the bronchi was significantly increased in the OA-challenged group (*P < 0.001 vs. Control group by unpaired Student's t-test).