Figure 6

Ex-vivo immune response in the prevention study (vaccination). A. In vitro stimulation of splenocytes from naïve mice with con A and CpG does not show any difference between B10.RIII (□) and IFN-β-/- mice (■). B. In vitro proliferation of OVA restimulated T cells from in vivo CpG-vaccinated OVA-primed B10.RIII (□) and IFN-β-/- mice (■). Mice were primed and challenged as in Figure 2. In vitro proliferation after recall with OVA was weaker in IFN-β-/- mice (■) than B10.RIII mice (□). C. Th-1 cytokines from supernatants after in vitro proliferation of OVA restimulated T cells in OVA-primed/CpG-vaccinated mice. IFN-γ, IL-12 and IL-2 production in supernatants from cell cultures was higher in B10.RIII than in IFN-β-/- mice. n = 5/group *P < 0.05 vs. OVA-treated B10.RIII mice.