Table 1 Association rate constant (K ass M-1sec-1) for inhibition of HNE by α1-AT with SP-A. SP-A employed was both from humans affected by PAP or from rabbit, polymyxin treated and polymyxin-mannose treated. Data are means ± SD of three different experiments.
Reaction conditions | Kass, (M-1sec-1) means ± SD | ||||
|---|---|---|---|---|---|
Human SP-A | Rabbit SP-A | ||||
α1-AT nM | SP-A nM | Native | Polymyxin-treated | Polymyxin/Mannose-treated | Native |
7.5 | 0 | 3.40 ± 0.0079 × 107 | 3.40 ± 0.0079 × 107 | 3.40 ± 0.0079 × 107 | 3.40 ± 0.0079 × 107 |
7.5 | 0.15 | 1.84 ± 0.0577 × 107 | 1.84 ± 0.0580 × 107 | 1.86 ± 0.0565 × 107 | 1.82 ± 0.0585 × 107 |
7.5 | 1.5 | 1.70 ± 0.0623 × 107 | 1.70 ± 0.0631 × 107 | 1.72 ± 0.0618 × 107 | 1.68 ± 0.0620 × 107 |
7.5 | 7.5 | 5.20 ± 0.0483 × 106 | 5.22 ± 0.0480 × 106 | 5.00 ± 0.0478 × 106 | 5.40 ± 0.0490 × 106 |
7.5 | 15 | 4.30 ± 0.0513 × 106 | 4.30 ± 0.0520 × 106 | 4.30 ± 0.0520 × 106 | 4.40 ± 0.0498 × 106 |