Figure 7
From: TGF-β1 and serum both stimulate contraction but differentially affect apoptosis in 3D collagen gels
TUNEL positivity in HFL-1 cells cultured in attached gels and Western blots for selected pro-apoptotic and anti-apoptotic factors. TUNEL Positivity. (A) Fibroblasts embedded in collagen gels which were left attached to the plates preventing contraction. After 5 days, gels were digested and stained for TUNEL. TUNEL positive cells were counted in 5 high-power fields and expressed as % of total cells. Data are presented as % of positive control (DNAse treated). Data are shown as means ± SEM. Western blot for selected pro-apoptotic and anti-apoptotic factors. (B) Collagen gels were digested, cells were collected, lysed and the cell lysate were evaluated by Western blot. Data presented are from one representative experiment.