Figure 3
From: Isolation of human β-defensin-4 in lung tissue and its increase in lower respiratory tract infection
Representative RP-HPLC profiles of hBD-4 immunoreactivity. Samples were obtained from 300 mg human lung tissue. Fraction volumes of 0.5 ml were obtained by RP-HPLC using a TSK ODS SIL 120A (4.6 Å × 150 mm) column and a linear gradient of 10–60% CH3CN containing 0.1% TFA at a rate of 1.0 ml/min for 40 min. Arrows indicate the elution position of synthetic hBD-4. "ir-hBD-4" on the Y-axis means immunoreactive hBD-4.