Analysis of nuclear NFκB recruitment by EMSA. Equal cell numbers from clones, indicated on the x-axis, were stimulated with 0.1 μg/ml LPS (A) or 5 ng/ml TNF-α (B) for one hour, before nuclear extracts were prepared. Specific bands were identified by supershift using a p65 antibody for NFκB and cold oligo competition. Bands were quantified by phosphorimager and expressed relative to that of the stimulated T clone, which was set at one. Data represent mean ± SEM from 2 independent experiments.