Figure 1

Production of reactive oxygen species (ROS) by bronchoalveolar lavage (BAL) cells, 1 day (A) and 14 days (B) after intranasal administration of bleomycin (0.1 mg, BLM) or saline (Control) to mice with the p47^phox subunit of NADPH-oxidase deleted (p47^phox -/- knockout mice (KO): solid bars), compared with "Wild Type" p47^phox +/+ mice (WT: blank bars). Cells were stimulated in vitro with PMA (0.8 μM) and ROS production was evaluated by chemiluminescence. Data were collected at the time of maximum light emission. Results are expressed as relative light units (mean ± SEM). ***: p < 0.001 comparison with control mice exposed to saline solution alone. ###: p < 0.001 for KO mice compared with WT mice. n = 5–9.