From: Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells
NOS isoform | Primer sequence |
---|---|
Rat iNOS [26] | sense:5'-GGACCACCTCTATCAGGAA-3', antisense 5'-CCTCATGATAACGTTTCTGGC-3'; |
Rat eNOS [27] | sense: 5'-TACCAGCCGGGGGACCAC-3', antisense: 5'-CGAGCTGAC-AGAGTAGTA-3'. |
Human iNOS [28] | sense: 5'-GAGCTTCTACCT-CAAGCTATC-3', antisense: 5'-CCTGATGTTGCCATTGTTGGT-3'; |
Human eNOS | sense:5'-GCACAGGAA-ATGTTCACC TAC-3', antisense: 5'-CACGATGGTGAC-TTTGGCTAG-3'. |
Mouse iNOS (real time PCR) probe | sense: 5'-CAGCTGGGCTGTACAAACCTT-3', antisense: 5'-CATTGGAAGTGAAGCGTTTCG-3', 5'-6FAM (fluorescent reporter dye, 6-carboxyfluorescein)-CGGGCAGCCTGTGAGACCTTTGA-TAMRA (quenching agent, 6-carboxytetramethylrhodamine, Applied Biosystems, CA, USA). |