Inhibition of TNFalpha in vivo prevents hyperoxia-mediated activation of caspase 3 in type II cells

Respiratory Research

Table 7 Effect of hyperoxia on apoptotic parameters in TIIcells

	Control	Hyperoxia
Bcl-2 (n = 5)	100	96 ± 8
Bax (n = 5)	100	134 ± 28*
Cytochrome c (n = 5)	100	102 ± 15
Mitochondrial membrane potential	100	81 ± 8*
Early apoptotic TIIcells	100	101 ± 26
Late apoptotic TIIcells	100	94 ± 22
Cell death detection ELISA	100	104 ± 13

Several apoptotic parameters were determined in freshly prepared TIIcells of rats exposed to air (control) or oxygen for 48 hrs (hyperoxia) as described in Materials and Methods. Values are means ± S.D. given in arbitrary units (control = 100) of n = 3 independent experiments unless stated otherwise. Asterisk indicates a significant difference to control (p < 0.05).

ISSN: 1465-993X