CpG-ODN-stimulation decreases apoptosis in HeLa and A549 cells. Cells were stained with Annexin-V after CpG-ODN-stimulation in the presence or absence of TNF-α and CHX after 24 h (A). Data are expressed as the mean ± SD (n = 6). Student's t test was used for statistical analysis. Representative histograms are shown from experiments with HeLa cells after CpG-ODN-stimulation in the absence (B) or presence (C) of TNF-α and CHX. Caspase 3 expression in HeLa cells is shown after incubation with TNF-α and CHX (D). In the presence of CpG-ODN the expression is decreased (E). The percentage of positive cells in each sample is indicated.