Figure 3
From: Application of microarray technology in pulmonary diseases
Image from laser scanning confocal microscope of a DNA microarray slide. mRNA, is extracted from a sample and a control and after its transcription into more stable cDNA, one is labeled with cy-5 (red fluorescent dye) and the other with cy-3 (green fluorescent dye). The two cDNA populations are allowed to hybridize to the same microarray slide. If particular mRNA from the sample is in abundance, the spot with a complementary probe will be red (induction of gene expression in sample condition); if the concentration of the particular mRNA is higher in the control, the spot will be green (induction of gene expression in control condition). If both samples contain the same amount of a given mRNA the spot will be yellow (equal gene expression in both conditions). (Adapted with permission of Dr Karameris Andreas.)