Figure 8

Phosphorylation (A, B) and degradation (C, D) of IκB-α in lung tissue. The groups represent rats treated as follows: Saline, saline (IT); LPS, LPS (IT); LPS-SP600125, LPS (IT) and a pretreatment with SP600125 (IO), Saline-SP600125, saline (IT) and a pretreatment with SP600125 (IO); LPS-PD98059, LPS (IT) and a pretreatment with PD98059 (IO), Saline-PD98059, saline (IT) and a pretreatment with PD98059 (IO). Animals were sacrificed 4 hours after LPS treatment. Western blots with anti-serine phospho-IκBα (Ser32)/IκBα antibody were employed to monitor phosphorylated IκB-α and IκB-α. Densitometry of phospho-IκB-α/IκB-α bands is expressed in arbitrary densitometric units. Values represent means ± SEM of results from 5 rats in each group. * Significant differences compared with saline, p < 0.05, and + significant difference compared with LPS group, p < 0.05.