Phosphorylation of JNK (A, B), ERK (C, D)and p38 MAP kinase (E, F) in lung tissue 4 hours after saline or LPS treatment. The groups represent rats treated as follows: Saline, saline (IT); LPS, LPS (IT); LPS-SP600125, LPS (IT) and a pretreatment with SP600125 (IO), Saline-SP600125, saline (IT) and a pretreatment with SP600125 (IO);LPS-PD98059, LPS (IT) and a pretreatment with PD98059 (IO), Saline-PD98059, saline (IT) and a pretreatment with PD98059 (IO). Western blots with anti-phospho-JNK/JNK antibody, phospho-ERK/ERK antibody or phospho-p38 MAP kinase/p38 MAP kinase were employed in order to monitor JNK, ERK or p38 MAP kinase phosphorylation. Relative values for levels of phosphorylated JNK1/2, ERK1/2 or p38 MAP kinse normalized to JNK1/2, ERK1/2 or p38 MAP kinase are indicated below the gel. Results are representative results from 5 rats in each group.