Table 1 Summary of pollen diffusate proteins identified by micro C18 RP-HPLC and ESI.
Spot | Gel (Mr) | micro LC-ESI | Mass (calc) |
|---|---|---|---|
1 | 30,000 | Acidic Cyn d 1 isoallergen isoform 3 precursor | 28,378 |
2 | 30,000 | Acidic Cyn d 1 isoallergen isoform 4 precursor | 28,407 |
3 | 30,000 | Acidic Cyn d 1 isoallergen isoform 2/4 precursor | 28,391 |
4 | 30,000 | Acidic Cyn d 1 isoallergen isoform 2/4 precursor | 28,407 |
5 | 25,000 | Acidic allergen Cyn d 1 precursor | 28,391 |
6 | 25,000 | Acidic allergen Cyn d 1 precursor | 26,645 |
7 | 25,000 | Acidic allergen Cyn d 1 precursor | 26,645 |
8 | 35,000 | Acidic allergen Cyn d 1 precursor | 26,645 |
9 | 50,000 | Acidic Cyn d 1 isoallergen isoform 1 precursor | 26,663 |
10 | 50,000 | Acidic Cyn d 1 isoallergen isoform 1 precursor | 26,663 |
11 | 50,000 | Phosphoglucomutase, cytoplasmic (PGM2) | 63,002 |
12 | 35,000 | Acidic Cyn d 1 isoallergen isoform 1 precursor | 26,663 |
13 | 35,000 | L-Ascorbate peroxidase, cytosolic isozyme, maize | 27,295 |
14 | 35,000 | Acidic Cyn d 1 isoallergen isoform 1 precursor | 26,663 |
15 | 35,000 | Phosphoglucomutase, cytoplasmic 2 | 63,002 |
16 | 40,000 | Acidic Cyn d 1 isoallergen isoform 1 precursor | 26,663 |
17 | 40,000 | Triosephosphate isomerase, cytosolic (TIM) | 27,008 |
18 | 40,000 | Enolase 2 | 48,132 |
19 | 40,000 | Profilin 3 (ZmPRO3) | 14,228 |
20 | 50,000 | Pistilata homolog ScPI (Sanguinaria Canadensis) | 24,075 |
21 | 60,000 | Acidic Cyn d 1 isoallergen isoform 1 precursor | 26,663 |
22 | 70,000 | Acidic allergen Cyn d 1 precursor | 26,645 |
23 | 60,000 | Acidic allergen Cyn d 1 precursor | 26,645 |
24 | 60,000 | Acidic Cyn d 1 isoallergen isoform 2 precursor | 28,391 |
25 | 90,000 | Acidic allergen Cyn d 1 | 26,645 |
26 | 12,000 | Acidic allergen Cyn d 1 | 26,645 |
27 | 18,000 | Acidic allergen Cyn d 1 precursor | 26,645 |
28 | 50,000 | Acidic allergen Cyn d 1 precursor | 26,645 |
29 | 50,000 | Acidic allergen Cyn d 1 precursor | 26,645 |
30 | 50,000 | Enolase | 47,956 |