Figure 2

NAC reduces monocrotaline-induced pulmonary accumulation of ED-1 and OX-62 positive cells. Representative fluorescent images compare the presence of (A) ED-1 positive monocytes/macrophages (red fluorescence arrows) in control rat (1) and lung of MCT-treated rats (2). NAC treatment substantially reduced the number of ED-1 positive cells in the lungs of MCT-treated rats (3). Immunofluorescent labelling for α-smooth muscle actin (green) was used to indentify vascular smooth muscle cells (blue fluorescence: nuclei). Presence of (B) dendritic cells OX62 positive leucocytes (red fluorescence arrows) in lung of control rat (1) and MCT-treated rats (2). NAC treatment substantially reduced the number of dendritic cells in the lungs of MCT-treated rats (3). 4: Bar graphs are summary data for mean number of ED-1+ cells/field and OX62+ cells/adventitia (mean ± SEM). *P < 0.05 vs cont, ^#p < 0.05 vs MCT) (n = 20–30 per group).