Figure 7

Representative phase contrast images of mouse alveolar type II cells cultured under EMT-inducing conditions. Images of the cells 6 days after culture are shown. MOCK: cells only treated with transfection reagents. Con siRNA: cells transfected with non-targeting siRNA. miR-200c: cells transfected with synthesized miR-200c. miR-21-i: cells transfected with miR-21 inhibitor. 200c & 21-i: cells co-transfected with both synthesized miR-200c and miR-21 inhibitor. Note that the cells cultured under EMT-inducing conditions adopted their spindle-like shape with irregular processes, whereas cells transfected with synthesized miR-200c and/or miR-21 inhibitor adopted a cobblestone-like appearance and had granules (lamellar bodies). Scale bars: 20 μm.