miR-21 is increased in lung epithelial cells during experimental bleomycin-induced lung injury. (A) Representative flow cytometric analyses of CD45- lung cells from saline- and bleomycin-treated mice. (B) The percentage of each cell type within all CD45- lung cells. Note that bleomycin induced a decrease in the percentage of epithelial cells (Ep) and an increase in that of mesenchymal cells (Me) but no change in endothelial cells (En). (C) The relative expression of mRNA of lung epithelial marker genes (E-cadherin and SFTPC) or EMT marker genes (Vimentin, Zeb1 and Zeb2) is shown. (D) The relative expression of microRNAs is shown. The values are mean ± SEM (n = 6). The values relative to those of the saline-treated epithelial cells are presented. The data were analyzed by Student t test. * p < 0.05 versus epithelial cells in the saline group. † p < 0.05 between saline group and bleomycin group in each cell type. § p < 0.05 versus epithelial cells in the bleomycin group.