Effects of CSD and its subdomains on Normal and SSc monocyte migration in vitro. Normal monocytes (A) were isolated from healthy donors, SSc monocytes (C) were isolated from SSc patients. The migration of these cells and Normal monocytes pretreated with TGFβ (B) toward CXCL12 was quantified in the presence of 5 μM of CSD or the indicated subdomains of CSD as described in the Methods. The Antennapedia Internalization Sequence alone was routinely used as the Control peptide; when tested scrambled CSD attached to the Antennapedia Internalization Sequence gave similar results. Each symbol represents the results obtained with cells from an individual donor.