Figure 3
From: The dopamine D1receptor is expressed and facilitates relaxation in airway smooth muscle
Immunohistochemical detection of dopamine D 1 receptor expression in human and guinea pig airway smooth muscle. (A and C) Representative immunohistochemical staining of dopamine D1 receptor (A), and dopamine D5 receptor (C) in paraformaldehyde/glutaraldehyde-fixed human trachea. (B and D) Anti-rabbit IgG isotype negative control in serial section of human tracheal airway smooth muscle. (E and G) Representative immunohistochemical staining of dopamine D1 receptor (E), and dopamine D5 receptor (G) in paraformaldehyde-fixed guinea pig trachea. (F and H) Anti-rabbit IgG isotype negative control in serial section of guinea pig trachea. All sections were counterstained with hematoxylin. Calibration bar: 100 μm. ASM, airway smooth muscle; Epi, airway epithelium. Images are representative of at least 3 independent immunohistochemical analyses from human and guinea pig trachea.