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Figure 2 | Respiratory Research

Figure 2

From: Cigarette smoke enhances human rhinovirus-induced CXCL8 production via HuR-mediated mRNA stabilization in human airway epithelial cells

Figure 2

CSE, HRV, or HRV+CSE do not alter AUF-1, KHSRP and HuR expression in HBE cells. HBE cells were treated with medium (control), CSE, HRV or the combination for the times indicated. Total cell lysates were harvested and analyzed via immunoblotting. Membranes were probed with specific AUF-1 (A), KHSRP (B) or HuR (C) antibodies, then were subsequently stripped and re-probed with an antibody to GAPDH to ensure equal protein loading. Data are representative of 3 separate experiments. Relative expression levels of each protein of interest from the 3 experiments were assessed by densitometry.

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Respiratory Research

ISSN: 1465-993X

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