Figure 6
From: Pyocyanin-induced mucin production is associated with redox modification of FOXA2
PCN-deficient mutant has reduced ability to induce the goblet cell hyperplasia in mouse airways. C57BL6 mice (n = 8) were repeatedly challenged with 1 x 106 of wild-type PA PAO1 or the isogenic ∆phzS mutant on Day 1, 3, 5 and 7. Mouse lungs were harvested, fixed and paraffin-embedded for PAS staining and for IHC. (A) PAS staining and quantification of goblet cells after infection by PAO1 versus ∆phzS. (B) IHC and quantification of MUC5AC induction by PAO1 versus ∆phzS. (C) IHC and quantification of MUC5B induction by PAO1 versus ∆phzS mutant. PAS, MUC5AC and MUC5B-positive cells were quantified using the AxioVision Rel. 4.8 software. Five representative microscopic fields at 10x magnification from each mouse were quantified. Data represent the mean ± SD from all eight mice.