Figure 2
From: Pyocyanin-induced mucin production is associated with redox modification of FOXA2
PCN induces posttranslational modifications and degradation of FOXA2. FOXA2 proteins were immunoprecipitated from nuclear lysate of NCI-H292 cells previously exposed to PCN for indicated times and concentrations, using anti-FOXA2 monoclonal antibody immobilized on Protein A/G Agarose, and analyzed by western blots using specific antibodies. The experiments were repeated three times with similar results. One typical western blot is shown. Densitometry data represent the mean ± SD from all three experiments. (A-D) Time and concentration-dependent inhibition of FOXA2 expression by PCN. (E-F) Decreasing levels of FOXA2 is accompanied by increasing levels of nitrosylation and acetylation (E) and ubiquitination (F).