PAR-2 expression on lung tissue and T cells in PBLN. (A) PAR-2 stained lung tissue obtained 6hr and 48 hr after secondary challenge (final magnification:x400). (a) 6 hr: PBS/OVA/vehicle/OVA, (b) 48 hr:PBS/OVA/vehicle/OVA, (c) 6 hr: OVA/OVA/vehicle/OVA, (d) 6 hr:OVA/OVA/Sivelestat/OVA, (e) 48 hr: OVA/OVA/vehicle/OVA, (f) 48 hr: OVA/OVA/Sivelestat/OVA. (B) The number of PAR-2-positive cells. *Significant differences (P<0.05) between PBS/OVA/vehicle/OVA and OVA/OVA/vehicle/OVA. Data represent the mean ± SD (n = 6 in each group). (C) Dotted line: Control antibody, Thin line: anti-human PAR-2 mAb, Non/Non/Non, Bold line: anti-human PAR-2 mAb, OVA/OVA/OVA. PAR-2 expression of CD3+ and CD4+ T Cells in PBLN. (a) CD3+ T cells in the PBLN and (b) CD4+ T Cells in PBLN were assessed by intracellular staining. The data shown are representative of three independent experiments. Increased numbers of PAR-2 positive CD3+ and CD4+ T cells from the PBLN of the sensitized and challenged mice were observed.