Figure 1

Flow cytometry of sputum cells from 2 representative subjects. Panels A and C show representative plots of unstained sputum. A gate was selected as shown to select for neutrophils on the basis of middle-range size and granularity. Panels B and D show these selected cells stained with the markers CD45 (pan-leukocyte marker) and CD14 (monocyte/macrophage marker). The CD14^low, CD45^high cells (gate A) were selected as representing neutrophils and analysed for expression of neutrophil markers. Monocytes/macrophages (CD14^high, CD45^high (gate B)) and epithelial cells (CD45^low, CD14^low cells (gate C)) were excluded.