Figure 8

RSV-infected BDCA-1⁺and BDCA-3⁺mDCs impair CD4⁺T cell proliferation. BDCA-1⁺ and BDCA-3⁺ mDC were incubated with RSV, UV-RSV, poly I:C or media for 24 hours. Cells were washed and cocultured with CFSE-labeled allogenic CD4⁺ T cells for 6 days, ( A ) On day 6, cells were stained with mAb against CD3 and CD4, and T cell proliferation was measured by examining CFSE expression on CD3⁺, CD4⁺ T cells. Data is from one donor and is representative of data from 3 donors. Bar graphs represent the percent of proliferating CD4⁺ CD3⁺ T cells after 6 days in coculture with untreated or treated (B) BDCA-1⁺ and (C) BDCA-3⁺ mDCs. Data represents the mean + SEM of the percent of proliferating T cells from 3 donors. *= Statistically significant (p≤0.05) difference in the percent of proliferating T cells after coculture with infected and uninfected cells as calculated by students paired t-test.