Figure 3

RSV infection induces upregulation of costimulatory molecules on BDCA-1⁺and BDCA-3⁺mDCs. BDCA-1⁺ and BDCA-3⁺ mDCs were incubated in media alone or infected with RSV at a MOI=5 for 40 h. ( A ) Costimulatory molecule expression was analyzed using flow cytometry. Dashed line= uninfected cells, solid line = infected cells. Data is from one donor and is representative of 5 donors. ( B ) Bar graph represent the change in mean fluorescent intensity (MFI) of expression by infected cells over uninfected cells (mean + SEM of 5 donors). Linear data is reported on a logarithmic scale. The dotted line represents a 2 fold induction. *=statistically significant difference (p≤0.05) in expression by infected mDCs as compared to uninfected mDCs by student’s paired t-test.