Figure 7

FOXJ1-RFX3 auto-regulatory feedback. A. Firefly luciferase activity in basal cells transfected with control EGFP, FOXJ1, RFX3 or FOXJ1+ RFX3 expression plasmids together with firefly luciferase reporter gene plasmids driven by the FOXJ1 and RFX3 promoters. The data were normalized for transfection efficiency per well by Renilla luciferase activity from co-transfected Renilla luciferase plasmid (pTK-RL) 48 hour after transfection. Bars represent mean ± standard error of pooled data from a minimum of three individual experiments assessed for 48 hr after transfection. B. TaqMan quantitative real-time RT-PCR assessment of the relative endogenous FOXJ1 mRNA expression in EGFP or RFX3-transfected basal cells for 48 hr after transfection. Bars represent mean ± standard error of pooled data from replicates of three individual experiments from different subjects.