Figure 6
From: RFX3 Modulation of FOXJ1 regulation of cilia genes in the human airway epithelium
Human FOXJ1 and RFX3 protein-to-protein interaction. 293A cells were transfected with FOXJ1 and RFX3 expression plasmids. After 48 hr, cell lysates were prepared in lysis buffer and incubated overnight with Anti-FLAG M2 affinity gel. Input and immunoprecipitated fractions were resolved by SDS-PAGE and analyzed by Western blotting with antibody specific for FOXJ1, FLAG and GAPDH as a loading control. Lanes 1-4 - Before immunoprecipitation (input). Lanes 5-8 - After RFX3 targeted immunoprecipitation. Lane 1 - PGK.EGFP and CMV.empty (mock); lane 2 - PGK.FOXJ1 and CMV.empty (FOXJ1); lane 3 - CMV.FLAG-RFX3 and PGK.EGFP (RFX3); lane 4 - PGK.FOXJ1 and CMV.FLAG-RFX3 (FOXJ1 + RFX3); lane 5 - PGK.EGFP and CMV.empty (mock); lane 6 - PGK.FOXJ1 and CMV.empty (FOXJ1); lane 7 - CMV.FLAG-RFX3 and PGK.EGFP (RFX3); and lane 8 - PGK.FOXJ1 and CMV.FLAG-RFX3 (FOXJ1 + RFX3).