Figure 5

RFX3-mediated enhancement of FOXJ1-induced expression of ciliated cell-associated genes in primary human basal cells transfected with both a FOXJ1 and RFX3 expression plasmid. A. TaqMan quantitative real-time RT-PCR assessment of the relative mRNA expression of the ciliated cell-associated genes DNAI1, DNALI1, SPAG6, TEKT1 and TEKT2 in basal cells transfected with 50% and 100% FOXJ1 expression plasmids compared to basal cells co-transfected with 50% and 100% of FOXJ1 and RFX3 expression plasmids together for 48 hr after transfection. B. TaqMan quantitative real-time RT-PCR assessment of the basal cell markers KRT5, KRT14 and TP63. C. Firefly luciferase activity assessment of basal cells transfected with firefly luciferase reporter gene plasmids driven by promoters of the ciliated cell-associated genes DNALI1, DNAI1, SPAG6 and TEKT1; or the basal cell marker KRT14; or a random sequence as a negative control. Data were normalized for transfection efficiency per well by Renilla luciferase activity from co-transfected Renilla luciferase plasmid (pTK-RL) 48 hr after transfection. Bars represent mean ± standard error of pooled data from replicates of a minimum of three individual experiments from different subjects.