Figure 4

Expression of cilia-associated transcription factor regulatory factor X3 (RFX3) in basal cells after transfection with a FOXJ1 or RFX3-expression plasmid. A. TaqMan quantitative real-time RT-PCR assessment of the relative endogenous RFX3 mRNA expression in EGFP or FOXJ1-transfected basal cells 48 hr after transfection. B. Firefly luciferase activity in basal cells transfected with control EGFP or FOXJ1 expression plasmids together with firefly luciferase reporter gene plasmid driven by the RFX3 promoter 48 hr after transfection. C. TaqMan quantitative real-time RT-PCR assessment of the relative RFX3 mRNA expression in basal cells transfected with the control EGFP or RFX3 expression plasmid. D. Western analysis assessment of RFX3 and GAPDH expression in untransfected basal cells and cells transfected with either control EGFP or RFX3 expression plasmid. Data represents the mean ± standard error of pooled data from replicates of a minimum of three individual experiments with cells from three different subjects.