Figure 2

Expression of the transcription factor FOXJ1 in primary human basal cells after transfection with a FOXJ1-expression plasmid. A. TaqMan quantitative real-time RT-PCR assessment of the relative FOXJ1 mRNA expression in untransfected basal cells (mock), compared to increasing amounts EGFP-transfected or FOXJ1-transfected basal cells. B. Western analysis assessment of FOXJ1 and GAPDH expression in untransfected basal cells and cells transfected with either the control EGFP or FOXJ1 expression plasmids. Data represents the mean expression level ± standard error of pooled data from three replicated individual experiments with cells from three different subjects. All assays were carried out 24 hr after transfection.