Figure 6

PI3K/Akt signaling pathway is required for uPAR-mediated EMT in HSAEpiC Cells. (A) Cells were cultured for 72 h without or with 5% CSE. Cell extracts were subjected to Western blot to detect phosphorylated Akt and total Akt. (B) Parental cells, empty vector and shuPAR2 cells were cultured for 72 h without or with 5% CSE. Cell extracts were subjected to Western blot analysis for phosphorylated Akt (p-Akt), phosphorylated GSK-3β (p-GSK-3β), Snail, and β-actin. (C) Cells were treated with 10 μM of the PI3K inhibitor LY294002 or with vehicle (control) for 72 h without or with 5% CSE. Cell extracts were subjected to Western blot analysis to detect p-Akt, p-GSK-3β, Snail, E-cadherin, α-SMA and β-actin (representative of three studies).