uPAR is required for CSE-induced EMT in HSAEpiC cells. (A) Cells expressing empty vector, shuPAR1 cells, and shuPAR2 cells were cultured for 24 h. UPAR mRNA was determined by Real-time PCR (mean ± SEM; n = 3). (B) uPAR protein level was determined by Western blot. (C) Empty vector and shuPAR2 cells were cultured for 72 h with 5% CSE. Cell images were captured by phase-contrast microscopy. (D) Parental cells, empty vector and shuPAR2 cells were cultured for 72 h without or with 5% CSE. Cell extracts were subjected to Western blot analysis for epithelial markers E-cadherin and α-catenin, mesenchymal markers N-cadherin and α-SMA. β-actin was used as a loading control.