Figure 2

Inflammatory response with high-pressure ventilation. High pressure ventilation results in an increase in the activation of NFκB, measured as the percentage of p65 positive nuclei (A) or as the levels of p65 protein content in nuclear extracts (B). Both clarithromycin and levofloxacin blocked this activation. Panel C shows representative p65-immunostained sections and western blots of nuclear extracts. Some positive nuclei are shown (arrowheads). Cxcl2 expression (D) and interleukin-10 levels were measured (E). There was a significant increase in Cxcl2 expression and a slight decrease in IL-10 levels with injurious ventilation in all treatment groups. *p < 0.05 in post-hoc tests compared to low-pressure ventilated counterparts.