Figure 4

Effects of MaR1 pretreatment on HDE-induced PKCα, PKCϵ, and PKA activities following treatment. BEAS-2B cells were pretreated with 0, 100, or 200 nM MaR1 for 1 hr, then treated with 5% HDE. At 1 hr or 6 hr post HDE treatment, cells were flash frozen, then assayed for PKCα (1 hr; A), PKCϵ (6 hr; B), and PKA (1 hr; C) activities, as previously described. #p < 0.05 vs. media; *p < 0.05 vs. 5% HDE; **p < 0.01 vs. 5% HDE; ***p < 0.001 vs. 5% HDE. PKA and PKC isoform-specific activities experiments were performed 3 separate times.