Table 2 Primers used in qRT-PCR amplification
Official gene symbol | Primer sequences (5′to 3′) | Primerbank ID | Product size | Efficiency |
|---|---|---|---|---|
ACTB (Ref. Gene) | Fwd: CTCTTCCAGCCTTCCTTCCT | N/A | 116 bp | 100% |
Rev: AGCACTGTGTTGGCGTACAG | ||||
ADM | Fwd: ATGAAGCTGGTTTCCGTCG | 4501945a1 | 112 bp | 100% |
Rev: GCCCACTTATTCCACTTCTTTCG | ||||
AQP9 | Fwd: CTGCAACCGTCTTTGGCATTT | 10280624a3 | 118 bp | 106% |
Rev: AGATACGGAGCTGGGTATGTT | ||||
AREG | Fwd: GTGGTGCTGTCGCTCTTGATA | 4502199a1 | 171 bp | 97% |
Rev: ACTCACAGGGGAAATCTCACT | ||||
GAPDH (Ref. Gene) | Fwd: CATGAGAAGTATGACAACAGCCT | 7669492a3 | 113 bp | 97% |
Rev: AGTCCTTCCACGATACCAAAGT | ||||
GJA1 (Connexin 43) | Fwd: GTGCCTGAACTTGCCTTTTC | N/A | 165 bp | 98% |
Rev: CCCTCCAGCAGTTGAGTAGG | ||||
IGFBP3 | Fwd: AGAGCACAGATACCCAGAACT | 4504617a2 | 105 bp | 100% |
Rev: TGAGGAACTTCAGGTGATTCAGT | ||||
NDRG1 | Fwd: TCGAGACTTTACATGGCTCTGT | 207028746b2 | 93 bp | 106% |
Rev: TCATGCCGATGTCATGGTAGG | ||||
SCGB1A1 (Uteroglobin) | Fwd: TTCAGCGTGTCATCGAAACCC | 4507809a1 | 189 bp | 100% |
Rev: ACAGTGAGCTTTGGGCTATTTTT | ||||
SPAG6 | Fwd: GTAAGGTGCTGCCGCATGATA | 6912678a1 | 152 bp | 100% |
Rev: CCTCACTATTTCCTCGGGGTA | ||||
TEKT1 | Fwd: CAGATTCGGATGAACCGCTCT | 16753231a3 | 140 bp | 103% |
Rev: CTCACGGCGTTCTCAGAATATC | ||||
TMEM45a | Fwd: GTTCACTTCCTGTGTCCTTAACC | 8922242b2 | 95 bp | 97% |
Rev: CATTTCCCGGCCATGAGTGT |