Figure 3

Comparison of Microarray and qRT-PCR expression data for selected genes. Comparison of Log₂ (fold change) of gene expression in CF vs. Control nasal cell samples as measured by microarray analysis (black) and qRT-PCR (grey) using independent samples (n = 6 CF and 5 Ctrl in each case). Microarray data are log₂(fold change) of normalized intensities, and qRT-PCR data are log₂(mean fold change). Direction of differential expression was in agreement for all genes except TMEM45a, whose up-regulation in CF was not supported.