Figure 3

The changes of Bcl-2 and Bcl-xL expression levels by AIM in alveolar macrophages. (A) Western blotting (WB) using anti-AIM of conditioned medium from control empty vector (lane 1) or AIM expression vector (lane 2) transfected HEK293 cells. Conditioned medium was collected after 48 h incubation. (B) WB using anti-Bcl-2, anti-Bcl-xL, and anti-β-actin of cell lysates of alveolar macrophages (AM) treated with control conditioned medium (lane 1), control conditioned medium containing CSE (1.0%) (lane 2), and conditioned medium containing AIM (lane 3)(upper panel). Cell lysates of AM were collected after 24 h treatment. Shown is a representative experiment of 3 showing similar results. The middle panel is the average (±SEM) of relative changes in Bcl-2 normalized to β-actin. The lower panel is the average (±SEM) of relative changes in Bcl-xL normalized to β-actin. Open bar is control conditioned medium treated, filled bar is control conditioned medium treated in the presence of CSE (1.0%), and horizontal crosshatched bar is conditioned containing AIM treated. *p < 0.05.