Figure 2

AIM expression in alveolar macrophages isolated from bronchoalveolar lavage fluid. (A) AM were treated with indicated concentrations of cigarette smoke extract (CSE) for 16 h (n = 5). Real time-PCR was performed using primers to AIM or β-actin, as a control. AIM expression was normalized to β-actin. Shown is the fold increase (±SEM) relative to control treated cells. *p < 0.05. (B) AM were treated with CSE (1.0%) and RNA were harvested at indicated time points (n = 4). Real time-PCR was performed using primers to AIM or β-actin. AIM expression was normalized to β-actin. Shown is the fold increase (±SEM) relative to control cells. *p < 0.05. (C) Western blotting (WB) using anti-AIM and anti-β-actin of cell lysates from indicated concentrations of CSE treated AM (upper panel). Cell lysates were collected after 16 h treatment. Shown is a representative experiment of 3 showing similar results. AIM was normalized to β-actin. The lower panel is the average (±SEM) of relative changes to control cells. (D) WB using anti-AIM and anti-β-actin of cell lysates from CSE (1.0%) treated AM (upper panel). Cell lysates were collected at indicated time points. Shown is a representative experiment of 3 showing similar results. AIM was normalized to β-actin. The lower panel is the average (±SEM) of relative changes to control cells.