SP-C deficiency increases the type II cell cytokine response to RSV infection and TLR3 challenge. Cytokine levels were measured in the culture media of Sftpc +/+ and -/- type II cells challenged with the TLR3 ligand poly I:C (Panel A) or by RSV infection (Panel B). Basal levels of cytokine expression were elevated in the media from Sftpc -/- type II cells and the response to poly I:C or virus was increased relative to the Sftpc +/+ cells. Cytokine concentrations are in pg/ml (x axis). N = 3.